高通量测序分析20株禽流感病毒全基因

doi:10.11843/j.issn.0366-6964.2016.07.018

Abstract

Abstract:

To discuss the sequencing and analysis of avian influenza virus (AIV) genome by high-throughput sequencing，genomes of the 20 AIV strains isolated from eastern China were sequenced by Ion Torrent PGM.The advantage and disadvantage of the method were evaluated.The characteristic of genome and evolution were analyzed.The molecular evolution relationships of the other 6 internal genes were also analyzed.The results showed that the whole genomes of all the 20 strains were completely sequenced by Ion Torrent PGM.All the 20 viruses are restricted to h9.4.2.5 clade of H9 subtype low pathogenic strains.NA genes of all the viruses are N2 subtype.High-throughput sequencing can be used in the genome analysis of AIV strains.

CLC Number:

S852.659.5

WANG Kai-cheng，ZHUANG Qing-ye，ZHANG Xiao-chun，QIU Yuan，WANG Tong，HOU Guang-yu，LIU Shuo，WANG Su-chun. Genome Analysis of the 20 Avian Influenza Virus Strains by High-Throughput Sequencing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1443-1450.

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Genome Analysis of the 20 Avian Influenza Virus Strains by High-Throughput Sequencing

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